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mouse renal cancer cell line renca  (Procell Inc)

 
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    Procell Inc mouse renal cancer cell line renca
    NFAT1 increases PD-L1 expression via upregulation of TNF expression in RCC cells. A IHC Images of NFAT1 and PD-L1 staining using TMA tissue sections ( n = 96 RCC). The scale bars were shown as indicated. B and C. Heatmap ( B ) and dot plot ( C ) to show the correlation of IHC scores for the expression of the PD-L1 and NFAT1 proteins in RCC patient specimens. ( r = 0.5021 for spearman correlation coefficients, P < 0.001). D The GEPIA web tool was searched for the correlation between the expression of PD-L1 and NFAT1 in mRNA levels in RCC samples. P values as indicated in the Fig. E-G. Western blot ( E ), qRT-PCR ( F ) and FACS analysis ( G ) of PD-L1 expression in renal cancer cells infected with shControl or shNFAT1s. GAPDH served as an internal reference. For qRT-PCR analysis, data presented as the mean ± SD of three independent experiments. ***, P < 0.001. H and I. Western blot ( H ) and qRT-PCR ( I ) analysis of PD-L1 expression in renal cancer cells infected with EV or NFAT1 plasmids. GAPDH served as an internal reference. For qRT-PCR analysis, data presented as the mean ± SD of three independent experiments. ***, P < 0.001. The differences were compared between shControl group and shNFAT1 group. J After 72 h of selection with puromycin, 5 × 10 6 <t>Renca</t> cells infected with shControl or shNFAT1 were subcutaneously injected into the right dorsal flank of C57BL/6 mice. Mice with subcutaneous Renca tumors ( n = 5/group) were treated with anti-PD-1 (200 μg) or nonspecific IgG for three times at day 2, 4, and 7. The mean of each group was compared with the mean of every other group. K Immunofluorescence staining analysis of the percentage of CD3 + CD4 + and CD3 + CD8 + T cells infiltrated in Renca tumors. Data are presented as the mean ± SD of five independent experiments (***, P < 0.001). L ChIP-qPCR of TNF in 786-O <t>and</t> <t>ACHN</t> cells. All data are shown as the mean values ± SD from three replicates. ns not significant; *** p < 0.001, unpaired t test. The difference was compared between IgG group and NFAT1 group, or shControl group and shNFAT1 group. M 786-O cells were infected or transfected with indicated constructs. Then these cells were treated with or without TNF-α neutralized antibody (50 pg/ml) for 24 h. qRT-PCR analysis of PD-L1 expression in 786-O and ACHN cells. GAPDH served as an internal reference. Data presented as the mean ± SD of three independent experiments. ***, P < 0.001. The mean of each group was compared with the mean of every other group.
    Mouse Renal Cancer Cell Line Renca, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse renal cancer cell line renca/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    mouse renal cancer cell line renca - by Bioz Stars, 2026-04
    90/100 stars

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    1) Product Images from "A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression"

    Article Title: A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/s13046-022-02253-0

    NFAT1 increases PD-L1 expression via upregulation of TNF expression in RCC cells. A IHC Images of NFAT1 and PD-L1 staining using TMA tissue sections ( n = 96 RCC). The scale bars were shown as indicated. B and C. Heatmap ( B ) and dot plot ( C ) to show the correlation of IHC scores for the expression of the PD-L1 and NFAT1 proteins in RCC patient specimens. ( r = 0.5021 for spearman correlation coefficients, P < 0.001). D The GEPIA web tool was searched for the correlation between the expression of PD-L1 and NFAT1 in mRNA levels in RCC samples. P values as indicated in the Fig. E-G. Western blot ( E ), qRT-PCR ( F ) and FACS analysis ( G ) of PD-L1 expression in renal cancer cells infected with shControl or shNFAT1s. GAPDH served as an internal reference. For qRT-PCR analysis, data presented as the mean ± SD of three independent experiments. ***, P < 0.001. H and I. Western blot ( H ) and qRT-PCR ( I ) analysis of PD-L1 expression in renal cancer cells infected with EV or NFAT1 plasmids. GAPDH served as an internal reference. For qRT-PCR analysis, data presented as the mean ± SD of three independent experiments. ***, P < 0.001. The differences were compared between shControl group and shNFAT1 group. J After 72 h of selection with puromycin, 5 × 10 6 Renca cells infected with shControl or shNFAT1 were subcutaneously injected into the right dorsal flank of C57BL/6 mice. Mice with subcutaneous Renca tumors ( n = 5/group) were treated with anti-PD-1 (200 μg) or nonspecific IgG for three times at day 2, 4, and 7. The mean of each group was compared with the mean of every other group. K Immunofluorescence staining analysis of the percentage of CD3 + CD4 + and CD3 + CD8 + T cells infiltrated in Renca tumors. Data are presented as the mean ± SD of five independent experiments (***, P < 0.001). L ChIP-qPCR of TNF in 786-O and ACHN cells. All data are shown as the mean values ± SD from three replicates. ns not significant; *** p < 0.001, unpaired t test. The difference was compared between IgG group and NFAT1 group, or shControl group and shNFAT1 group. M 786-O cells were infected or transfected with indicated constructs. Then these cells were treated with or without TNF-α neutralized antibody (50 pg/ml) for 24 h. qRT-PCR analysis of PD-L1 expression in 786-O and ACHN cells. GAPDH served as an internal reference. Data presented as the mean ± SD of three independent experiments. ***, P < 0.001. The mean of each group was compared with the mean of every other group.
    Figure Legend Snippet: NFAT1 increases PD-L1 expression via upregulation of TNF expression in RCC cells. A IHC Images of NFAT1 and PD-L1 staining using TMA tissue sections ( n = 96 RCC). The scale bars were shown as indicated. B and C. Heatmap ( B ) and dot plot ( C ) to show the correlation of IHC scores for the expression of the PD-L1 and NFAT1 proteins in RCC patient specimens. ( r = 0.5021 for spearman correlation coefficients, P < 0.001). D The GEPIA web tool was searched for the correlation between the expression of PD-L1 and NFAT1 in mRNA levels in RCC samples. P values as indicated in the Fig. E-G. Western blot ( E ), qRT-PCR ( F ) and FACS analysis ( G ) of PD-L1 expression in renal cancer cells infected with shControl or shNFAT1s. GAPDH served as an internal reference. For qRT-PCR analysis, data presented as the mean ± SD of three independent experiments. ***, P < 0.001. H and I. Western blot ( H ) and qRT-PCR ( I ) analysis of PD-L1 expression in renal cancer cells infected with EV or NFAT1 plasmids. GAPDH served as an internal reference. For qRT-PCR analysis, data presented as the mean ± SD of three independent experiments. ***, P < 0.001. The differences were compared between shControl group and shNFAT1 group. J After 72 h of selection with puromycin, 5 × 10 6 Renca cells infected with shControl or shNFAT1 were subcutaneously injected into the right dorsal flank of C57BL/6 mice. Mice with subcutaneous Renca tumors ( n = 5/group) were treated with anti-PD-1 (200 μg) or nonspecific IgG for three times at day 2, 4, and 7. The mean of each group was compared with the mean of every other group. K Immunofluorescence staining analysis of the percentage of CD3 + CD4 + and CD3 + CD8 + T cells infiltrated in Renca tumors. Data are presented as the mean ± SD of five independent experiments (***, P < 0.001). L ChIP-qPCR of TNF in 786-O and ACHN cells. All data are shown as the mean values ± SD from three replicates. ns not significant; *** p < 0.001, unpaired t test. The difference was compared between IgG group and NFAT1 group, or shControl group and shNFAT1 group. M 786-O cells were infected or transfected with indicated constructs. Then these cells were treated with or without TNF-α neutralized antibody (50 pg/ml) for 24 h. qRT-PCR analysis of PD-L1 expression in 786-O and ACHN cells. GAPDH served as an internal reference. Data presented as the mean ± SD of three independent experiments. ***, P < 0.001. The mean of each group was compared with the mean of every other group.

    Techniques Used: Expressing, Staining, Western Blot, Quantitative RT-PCR, Infection, Selection, Injection, Immunofluorescence, ChIP-qPCR, Transfection, Construct



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    NFAT1 increases PD-L1 expression via upregulation of TNF expression in RCC cells. A IHC Images of NFAT1 and PD-L1 staining using TMA tissue sections ( n = 96 RCC). The scale bars were shown as indicated. B and C. Heatmap ( B ) and dot plot ( C ) to show the correlation of IHC scores for the expression of the PD-L1 and NFAT1 proteins in RCC patient specimens. ( r = 0.5021 for spearman correlation coefficients, P < 0.001). D The GEPIA web tool was searched for the correlation between the expression of PD-L1 and NFAT1 in mRNA levels in RCC samples. P values as indicated in the Fig. E-G. Western blot ( E ), qRT-PCR ( F ) and FACS analysis ( G ) of PD-L1 expression in renal cancer cells infected with shControl or shNFAT1s. GAPDH served as an internal reference. For qRT-PCR analysis, data presented as the mean ± SD of three independent experiments. ***, P < 0.001. H and I. Western blot ( H ) and qRT-PCR ( I ) analysis of PD-L1 expression in renal cancer cells infected with EV or NFAT1 plasmids. GAPDH served as an internal reference. For qRT-PCR analysis, data presented as the mean ± SD of three independent experiments. ***, P < 0.001. The differences were compared between shControl group and shNFAT1 group. J After 72 h of selection with puromycin, 5 × 10 6 <t>Renca</t> cells infected with shControl or shNFAT1 were subcutaneously injected into the right dorsal flank of C57BL/6 mice. Mice with subcutaneous Renca tumors ( n = 5/group) were treated with anti-PD-1 (200 μg) or nonspecific IgG for three times at day 2, 4, and 7. The mean of each group was compared with the mean of every other group. K Immunofluorescence staining analysis of the percentage of CD3 + CD4 + and CD3 + CD8 + T cells infiltrated in Renca tumors. Data are presented as the mean ± SD of five independent experiments (***, P < 0.001). L ChIP-qPCR of TNF in 786-O <t>and</t> <t>ACHN</t> cells. All data are shown as the mean values ± SD from three replicates. ns not significant; *** p < 0.001, unpaired t test. The difference was compared between IgG group and NFAT1 group, or shControl group and shNFAT1 group. M 786-O cells were infected or transfected with indicated constructs. Then these cells were treated with or without TNF-α neutralized antibody (50 pg/ml) for 24 h. qRT-PCR analysis of PD-L1 expression in 786-O and ACHN cells. GAPDH served as an internal reference. Data presented as the mean ± SD of three independent experiments. ***, P < 0.001. The mean of each group was compared with the mean of every other group.
    Mouse Renal Cancer Cell Line Renca, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse renal cancer cell line renca/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    mouse renal cancer cell line renca - by Bioz Stars, 2026-04
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    Image Search Results


    NFAT1 increases PD-L1 expression via upregulation of TNF expression in RCC cells. A IHC Images of NFAT1 and PD-L1 staining using TMA tissue sections ( n = 96 RCC). The scale bars were shown as indicated. B and C. Heatmap ( B ) and dot plot ( C ) to show the correlation of IHC scores for the expression of the PD-L1 and NFAT1 proteins in RCC patient specimens. ( r = 0.5021 for spearman correlation coefficients, P < 0.001). D The GEPIA web tool was searched for the correlation between the expression of PD-L1 and NFAT1 in mRNA levels in RCC samples. P values as indicated in the Fig. E-G. Western blot ( E ), qRT-PCR ( F ) and FACS analysis ( G ) of PD-L1 expression in renal cancer cells infected with shControl or shNFAT1s. GAPDH served as an internal reference. For qRT-PCR analysis, data presented as the mean ± SD of three independent experiments. ***, P < 0.001. H and I. Western blot ( H ) and qRT-PCR ( I ) analysis of PD-L1 expression in renal cancer cells infected with EV or NFAT1 plasmids. GAPDH served as an internal reference. For qRT-PCR analysis, data presented as the mean ± SD of three independent experiments. ***, P < 0.001. The differences were compared between shControl group and shNFAT1 group. J After 72 h of selection with puromycin, 5 × 10 6 Renca cells infected with shControl or shNFAT1 were subcutaneously injected into the right dorsal flank of C57BL/6 mice. Mice with subcutaneous Renca tumors ( n = 5/group) were treated with anti-PD-1 (200 μg) or nonspecific IgG for three times at day 2, 4, and 7. The mean of each group was compared with the mean of every other group. K Immunofluorescence staining analysis of the percentage of CD3 + CD4 + and CD3 + CD8 + T cells infiltrated in Renca tumors. Data are presented as the mean ± SD of five independent experiments (***, P < 0.001). L ChIP-qPCR of TNF in 786-O and ACHN cells. All data are shown as the mean values ± SD from three replicates. ns not significant; *** p < 0.001, unpaired t test. The difference was compared between IgG group and NFAT1 group, or shControl group and shNFAT1 group. M 786-O cells were infected or transfected with indicated constructs. Then these cells were treated with or without TNF-α neutralized antibody (50 pg/ml) for 24 h. qRT-PCR analysis of PD-L1 expression in 786-O and ACHN cells. GAPDH served as an internal reference. Data presented as the mean ± SD of three independent experiments. ***, P < 0.001. The mean of each group was compared with the mean of every other group.

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression

    doi: 10.1186/s13046-022-02253-0

    Figure Lengend Snippet: NFAT1 increases PD-L1 expression via upregulation of TNF expression in RCC cells. A IHC Images of NFAT1 and PD-L1 staining using TMA tissue sections ( n = 96 RCC). The scale bars were shown as indicated. B and C. Heatmap ( B ) and dot plot ( C ) to show the correlation of IHC scores for the expression of the PD-L1 and NFAT1 proteins in RCC patient specimens. ( r = 0.5021 for spearman correlation coefficients, P < 0.001). D The GEPIA web tool was searched for the correlation between the expression of PD-L1 and NFAT1 in mRNA levels in RCC samples. P values as indicated in the Fig. E-G. Western blot ( E ), qRT-PCR ( F ) and FACS analysis ( G ) of PD-L1 expression in renal cancer cells infected with shControl or shNFAT1s. GAPDH served as an internal reference. For qRT-PCR analysis, data presented as the mean ± SD of three independent experiments. ***, P < 0.001. H and I. Western blot ( H ) and qRT-PCR ( I ) analysis of PD-L1 expression in renal cancer cells infected with EV or NFAT1 plasmids. GAPDH served as an internal reference. For qRT-PCR analysis, data presented as the mean ± SD of three independent experiments. ***, P < 0.001. The differences were compared between shControl group and shNFAT1 group. J After 72 h of selection with puromycin, 5 × 10 6 Renca cells infected with shControl or shNFAT1 were subcutaneously injected into the right dorsal flank of C57BL/6 mice. Mice with subcutaneous Renca tumors ( n = 5/group) were treated with anti-PD-1 (200 μg) or nonspecific IgG for three times at day 2, 4, and 7. The mean of each group was compared with the mean of every other group. K Immunofluorescence staining analysis of the percentage of CD3 + CD4 + and CD3 + CD8 + T cells infiltrated in Renca tumors. Data are presented as the mean ± SD of five independent experiments (***, P < 0.001). L ChIP-qPCR of TNF in 786-O and ACHN cells. All data are shown as the mean values ± SD from three replicates. ns not significant; *** p < 0.001, unpaired t test. The difference was compared between IgG group and NFAT1 group, or shControl group and shNFAT1 group. M 786-O cells were infected or transfected with indicated constructs. Then these cells were treated with or without TNF-α neutralized antibody (50 pg/ml) for 24 h. qRT-PCR analysis of PD-L1 expression in 786-O and ACHN cells. GAPDH served as an internal reference. Data presented as the mean ± SD of three independent experiments. ***, P < 0.001. The mean of each group was compared with the mean of every other group.

    Article Snippet: Two human renal cancer cell lines (786-O and ACHN) and a mouse renal cancer cell line (Renca) were periodically validated via short tandem repeat (STR) profiling (Procell, China).

    Techniques: Expressing, Staining, Western Blot, Quantitative RT-PCR, Infection, Selection, Injection, Immunofluorescence, ChIP-qPCR, Transfection, Construct